Amanda,

We use the following:


Amylase Reagent
Warning: Harmful, contains azide - see MSDS
Alpha Amylase from Bacillus Subtilis (Fluka Cat No 10070,)	1g
Oxoid PBS Tablets (Cat No BR14a)				1 tablet
Distilled water							100ml
Sodium Azide							0.1g
This solution, once prepared is stored at 4oC when not in use. A
recycled antibody dropper bottle (often used in commercial
immunoperoxidase kits) is useful for storage and application.

Reference:	 
V-M. Mangan, V. Farago, M. Kelly, and A. F. Henwood (2002) " An Amylase
Reagent with a Long Shelf Life for the Removal of Glycogen from Tissue
Sections" J Histotechnol. 25(3): 153-4.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garcia,
Amanda
Sent: Friday, 26 May 2006 4:27 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)



Can anyone help me out and tell me where you order diastase? Thanks in
advance.
> Amanda (Amy) Garcia
> Histology/Pathology
> College Station Medical Center
> (979) 680-5372 office
> (979) 696-5446 fax
> *Mailto:amanda.garcia@triadhospitals.com
> 
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