RE: [Histonet] hematoxylins ??

From:"Lee & Peggy Wenk"

Any hematoxylin powder without any other dye like Orange G (OG). The
hematoxylin Color Index is 75290, not 175290. Fisher, Polyscience, and Sigma
sell it, to name a few.

I have a couple of questions, though, if I could be so bold.
- how are you planning on disposing of the lead hematoxylin? It cannot be
disposed of down the sink, including all the water washing after hematoxylin
staining.
- have you consider immunohistochemistry for somatostatin? Much more
specific.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven
Coakley
Sent: Thursday, May 25, 2006 12:12 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] hematoxylins ??

Whats the best hematoxlyin powder/crystal to use in histology.  I purchased
a powder CI 175290 which contains OG powder ???.  In making up weigerts Iron
Hematoxylin I ended up using twice the recommended just to get the solution
to stain properly.  I will be making up a lead hematoxylin for pancreatic D
cells and am starting to think I need a different hematoxylin.
   
  Steve

		
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