RE: [Histonet] Ventana vs Dako- BUT prefer the BOND

From:"Tony Henwood"


We have a Bond Max and can't speak more highly of it. I have been doing
IPXs since 1979, using the old Sternberger's PAP Complex method and
later using ABC and then Polymer and unfortunately I had to concede that
my manual results were never as good as what the Bond's can produce now.
Results are consistent with no failed runs.

It might be that Novacastra is now part of the Vision group of companies
and their expertise and quality reagents have a lot to do with it.


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
[] On Behalf Of Andrea
C. Bilger
Sent: Wednesday, 31 May 2006 11:46 AM
Subject: [Histonet] Ventana vs Dako

I ave been reading with interest the mailings about the 2 top IP
stainers.  We have 2 Ventana XT's in our lab.  Have found them very
expensive to operate as everyone else has.  The Pathologists wanted us
to continue with Ventana due to only having one knowlegable IP tech and
wanting consistancy.  You have to have some knowlege of IP's even to
operate the Ventana machines.  Some reps make it sound goof proof but it
isn't.  We have had trouble with consistancy in a couple of detection
kits.  One was stronger and all our stains were too hot.  Adjusted the
stains and the next kit was back to the weaker strength.  Had to adjust
all the stains back again.  What a pain in the ......

Has anyone had any experience with another IP stainer from Vision
Biosystems?  I looked at one at a National Histo convention and it
sounded like a cross between the Dako and the Ventana.  I think it was
more of an open system but used bar codes on the slides.  Antigen
retrieval was done on the stainer. Reagents were supposed to be cheaper
and not proprietary.  It had a feature where you could start a stat
stain after the run was started.  I would be interested to hear anyones
experience with this machine.

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