RE: [Histonet] Sealing Paraffin Blocks

From:"Jackie M O'Connor"

Sealing also deters rodents and roaches from eating the blocks - I've seen 
this in the warmer parts of the US - I've worked at some pretty old 

"Rittman, Barry R"  
Sent by:
05/24/2006 10:32 AM



RE: [Histonet] Sealing Paraffin Blocks

I am a strong believer in sealing blocks.
While paraffin does penetrate into cells etc. it is still possible for
water (and organisms of various types) to penetrate into the block.
After all we use water to soak into some blocks to enhance cutting.
I have seen stored, unsealed blocks, with massive penetration of fungi
throughout the entire tissue. Also if unsealed blocks are stored in a
dry atmosphere they will tend to shrink. 
If you are going to store blocks for any length of time I would seal
them not just dipping  them into molten paraffin but melting the surface

-----Original Message-----
[] On Behalf Of Carole
Sent: Wednesday, May 24, 2006 9:21 AM
Subject: RE: [Histonet] Sealing Paraffin Blocks

They say if you seal the blocks they are good much longer for special
stains, etc.  We have a ring behind the block holder (from
that keeps everyone's microtome lined up the same.  You can also by the
Histo collimator (also from RA and I think Newcomer) to line up your
so you will not cut the tissue away on recuts.  It is a little more

Carole Fields, HT,ASCP
Pathology Supervisor
Lexington Medical Center
2720 Sunset Blvd.
W. Columbia, SC 2916

-----Original Message-----
From: Horn, Hazel V []
Sent: Wednesday, May 24, 2006 9:36 AM
Subject: RE: [Histonet] Sealing Paraffin Blocks

We used to do this years ago.  We no longer seal blocks.   In fact, I
believe you cut away more on a sealed block if the block requires

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
800 Marshall    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3912

visit us on the web at:

-----Original Message-----
[] On Behalf Of
Sent: Tuesday, May 23, 2006 5:45 PM
Subject: [Histonet] Sealing Paraffin Blocks

What is the current opinion and practice on sealing paraffin blocks
to filing/archiving them?  What is the pro and con in your opinion?
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