RE: [Histonet] Re: Bouins alternative/substitute question

From:"Bonner, Janet"

Very interesting!  I guess we'll have to give Gayle a break today......

________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of nienhuis@ucla.edu
Sent: Mon 5/22/2006 5:25 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Bouins alternative/substitute question



The article is available online if anyone wants to see it. 
 

Bob Nienhuis 
nienhuis@ucla.edu 

Quoting Carole Fields : 

> This is totally off the subject but.... I was reading an article in the May 
> 06 Smithsonian Magazine at my doctors office and noticed Gayle Callis's name 
> in the article about dinosaurs.  It was a wonderful article about some 
> really exciting new discoveries in Montana.   Everyone should read this 
> wonderful article.  Congratulations on being involved in this exciting 
> project. 
> Carole Fields 
> Lexington, SC 
> 
> 
> -----Original Message----- 
> From: Gayle Callis [mailto:gcallis@montana.edu] 
> Sent: Monday, May 22, 2006 12:34 PM 
> To: Kemlo Rogerson; Histonet@lists.utsouthwestern.edu 
> Subject: RE: [Histonet] Re: Bouins alternative/substitute question 
> 
> 
> Kemlo, 
> 
> Interesting that you said this, and also a story from our university 
> chemical safety handling of dry picric acid found in an old 
> laboratory.  They had a hard time getting it to explode also. 
> 
> Chemical safety removed a jar of picric acid,  dry and age 
> undetermined.  Knowing it was explosive, the fellow in charge took it out 
> to a very remote area and shot at the jar with a rifle, and it took a lot 
> of rifle bullets to get it to blow up.  He did have a good session of 
> target practice for deer hunting season being in the Wild Wild West of 
> Montana. 
> 
> The potential is there for explosion, and we still have picric acid on our 
> shelves, although stored under a generous layer of distilled water.  When 
> we need to make up Bouins, we just scoop it out and make sure we have a 
> saturated solution of picric acid. 
> 
> Care must be taken to keep dry crystals off lids, these are actually sealed 
> by dipping into hot paraffin, and check regularly for any leakage around 
> lid.  I don't think it would be a good thing to have in a laboratory fire, 
> but solvents are just as bad.  How many people still store isopentane in a 
> non explosion proof freezer - now that IS an explosive situation. 
> 
>  We remain cautious but not in panic state.  Bouins is still very 
> important as a fixative and mordant for Massons trichrome, we will continue 
> to use it, dispose of it correctly and not put metal cassettes into it.  We 
> often purchase it ready made from Sigma to avoid having to handle it. 
> 
> At 09:27 AM 5/22/2006, you wrote: 
>> I think it is because picric acid explodes when dry but I've tried, and 
>> tried, and tried and it has never exploded. 
>> 
>> Has anyone ever succeeded? 
>> 
>> Kemlo Rogerson 
>> Pathology Manager 
>> Ext  3311 
>> DD   01934 647057 
>> Mob 07749 754194 
>> 
>> Education is not the filling of a pail, but the lighting of a fire. --W. B. 
>> Yeats 
>> 
>> 
>> 
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> 
> Gayle Callis 
> MT,HT,HTL(ASCP) 
> Research Histopathology Supervisor 
> Veterinary Molecular Biology 
> Montana State University - Bozeman 
> PO Box 173610 
> Bozeman MT 59717-3610 
> 406 994-6367 
> 406 994-4303 (FAX) 
> 
> 
> 
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