Nalini
While the solution may not be good, how about fixing using vapor
fixation. There should still be sufficient formaldehyde vapor to fix the
cells and eliminates worrying about osmolarity etc of the solution.
Barry

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Makhijani, Nalini S
Sent: Wednesday, May 24, 2006 1:06 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Paraformaldehyde

Is 2 month old 4%PFA kept at room temp still good for the fixation of
cells on coverslips?

Thanks,

Nalini

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