RE: [Histonet] FW: Jb-4 blocks

From:"Edmondson David \(RBV\) NHS Christie Tr"

It's years ago, but I remember something along the lines of 70% alc, and
picking up the section with a brush in a droplet of the alcohol and
flicking it onto a staining pot of water.   They span and the creases
came out .  Then picked up onto slides and dried on a hotplate. 
Does that approach sound familiar to anyone?

Christie Hosp
Manchester UK

-----Original Message-----
[] On Behalf Of Allen,
Sent: 18 May 2006 22:45
Subject: [Histonet] FW: Jb-4 blocks

> Histonetter's,
> The following question was posted on the microscopy list serve by a 
> friend.  I thought maybe some of the folks in histoland might have 
> some good suggestions.
> Rhonda Allen
> "We are doing a rush job for a client who requires 4.0 um sections 
> from JB 4 blocks.  Our ultramicrotomists extraordinaire, Cheryl, is 
> having a dickens of a time getting the sections to remain flat when 
> removing them from the knife.  She is cutting on glass and taking 
> sections from the dry edge with a fine forceps.  As soon as the 
> sections leave the knife, they curl and won't uncurl when placed on a 
> drop of water on a slide.
> Not only is this a rush job in support of a grant proposal, but it 
> requires serial sectioning with no missing sections, and we have, 
> like,
> no real experience with this resin.   Cheryl has tried various sized
> block faces and different thicknesses for the sections, but nothing is

> helping.
> The thumping sound you hear is a head hitting a wall----repeatedly.
> Can anyone HEEEELLLLP??"
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