[Histonet] cytochrome c oxidase and succinate dehydrogenase
I have been asked to perform these enzyme stains on murine heart.
Since this is new to me, I'm asking the experts for any favorite
protocols. Even better, any commercially available kits.
I also have a question about the frozen sectioning. In the clinical
setting, we always froze the muscle biopsy without OCT. Is there a
good reason for this? I think that filling the heart with diluted
OCT will produce a nicer looking section, but I don't want to
compromise the staining.
Denise Crowley
Facility Manager Histology
Center for Cancer Research
Massachusetts Institute of Technology
40 Ames St. E17-230
Cambridge MA 02139
617-258-8183
dencrowl@mit.edu
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