[Histonet] cytochrome c oxidase and succinate dehydrogenase

From:Denise Crowley

I have been asked to perform these enzyme stains on murine heart.   
Since this is new to me, I'm asking the experts for any favorite  
protocols. Even better, any commercially available kits.

I also have a question about the frozen sectioning.  In the clinical  
setting, we always froze the muscle biopsy without OCT.  Is there a  
good reason for this?  I think that filling the heart with diluted  
OCT will produce a nicer looking section, but I don't want to  
compromise the staining.

Denise Crowley
Facility Manager Histology
Center for Cancer Research
Massachusetts Institute of Technology
40 Ames St. E17-230
Cambridge MA  02139

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