[Histonet] RE: Hoechst staining method
Some years ago, I looked into fluorescent nuclear counterstains,
including dapi, propidium iodide and HOECHST. Heochst is also known
Mix 1mg bisBemzimide (Sigma B2883) with 1ml Dist water. Take 4.0
micro litres of this stock and dilute with 1ml PBS pH 7.4.Apply to
sections for 10 mins, wash 3 x in PBS. Fluorescence is bright blue,
viewed with a UV filter 340 nm.
Latt,SA and Stetten GJ. Histochem. Cytochem 24,24 (1976)
Araki, T et al, Histochem. 87,331 (1987)
We are doing an immunofluorescence research project for podocytes.
The urine cytospins are to be counterstained with Hoescht. We have
never used it - can someone recommend a concentration, diluent and
staining time that we could start with. Thanks in advance.
Cardiovascular Research Unit
Div. of Cardiothoracic Surgery
Chris Barnard Building
University of Cape Town
Republic of South Africa
Tel +27 21 406-6589
Cel +27 82 469-3352
Fax +27 21 448-5935
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