[Histonet] Nerve staining
I have 10% NBF fixed porcine tissue embedded in paraffin and want to stain
for nerve fibers (specifically the celiac plexus). I have looked back at the
archives and understand using the silver stains like Bodian's can be rather
tricky so wanted to get peoples opinion on nerve staining.
My questions are:
1) Is there another 'less tricky' stain that can be used for nerve fibers
(methylene blue) ? Or a more user friendly method (Someone mentioned a
2) Any tips for getting over the tricky steps?
3) Has anyone tried the Bodian's 'kit' sold by Electron Microscopy Science?
Thanks in advance for the insight.
Applied Physics Laboratory
University of Washington
Seattle WA 98195
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