[Histonet] Feed back on bone fixation

From:"Reuel Cornelia"

We are fixing our bone with 70% alcohol for our research project, some
will be process for MMA(plastic) and the rest for paraffin embedding. We
will be transferring the bone tissue to 10% formalin for 24 hrs and
decalcify with EDTA for HE, Enzyme and Immunocytochemistry studies for
our paraffin embedded bone. We normally fixed the  tissue in 10%
formalin and transfer the rest of the tissue to 70% alcohol for storage.
But we never have done 70% alcohol to 10% formalin in any of our
paraffin embedded tissue. Any comments, theoretical explanation on the
effects of fixation will be helpful. 

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