I do mine at 4 um.

Jan Shivers
UMN VDL

----- Original Message ----- 
From: "Edwards, R.E." 
To: 
Sent: Thursday, May 18, 2006 5:43 AM
Subject: [Histonet] section thickness





In your  invaluable, but,  no  doubt differing opinions, what  is  the 
optimal section thickness   for formalin fixed paraffin processed tissues 
for  immunostaining, using  DAB  as  chromogen?, there has been  some 
discussion here  whether  it  should be 4,5,6, or  7microns, many thanks.

                                                                             
                   Richard  Edwards

                                                                             
                    MRC TOXICOLOGY UNIT
                                                                             
                             LEICESTER..U.K.......


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