Re: [Histonet] safranin o staining- glycosaminoglycans-cartilage

From:Gayle Callis

I will be attaching the safranin O/fast green method to you privately, the 
original publication was by Rosenberg, reference will be in 
protocol.  Rosenberg originally did this on frozen sections of articular 
cartilage and it has since been modified for formalin fixed, decalcified 
bone paraffin sections.  It is wise to include an undecalcified, normal 
piece of articular cartilage as a normal control to see the tinctorial 
quality compared to a decalcified cartilage to ensure decalcifiers have not 
affected the glycoaminoglycans.

At 05:22 AM 5/11/2006, you wrote:

>Dear Histonetters,
>                               I am in need of the procedure for staining of
>glycosaminoglycans  in articular cartilage. The literature search indicated
>the   use   of   safranin   o   staining   for   the   characterization  of
>glycosaminoglycans.  If any body have good experience in this procedure and
>able  to  share  their  protocol. I have already ordered a paper that deals
>with this.
>Method  of  Histomorphometric Assessment of Glycosaminoglycans in Articular
>Journal of Orthopaedic Research Vol 15, 670-674.  1977
>Choji Shimizu, Richard D. Coutts, Robert M. Healey Toshikazu Kubo, Yasusuke
>Hirasawa and David Amiel.

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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