Re: [Histonet] Marking prostate biopsies

From:"Joe Nocito"

I'm with Diane,
we also use eosin in our last 100% ETOH and haven't had problems and we have 
new graduates embedding biopsies.

Histology Manager
Pathology Reference Lab
San Antonio, TX

----- Original Message ----- 
From: "Gladney, Diane C Ms MACH" 
To: "Vickroy, Jim" ; 

Sent: Thursday, May 11, 2006 9:36 AM
Subject: RE: [Histonet] Marking prostate biopsies

I use Eosin in my last 100% Ethanol on my tissue processor. It works
great for the small biopsies of other tissue also. We have not had any
problems seeing these small biopsies or needle core biopsies since I
started doing this.


Diane C. Gladney, HT (ASCP)
Supervisor, Histology Dept.

Moncrief Army Community Hospital
Dept. of Pathology
4500 Stuart St.
FT. Jackson, SC  29207


Phone:  803-751-2530
FAX:     803-751-7829
DSN:    734-2530

-----Original Message-----
[] On Behalf Of Vickroy,
Sent: Thursday, May 11, 2006 10:29 AM
Subject: [Histonet] Marking prostate biopsies

We are looking for a way to better mark our small needle biopsies such
as breast cores and prostate biopsies so that they are more easily seen
after processing.

Right now the embedder has some trouble identifying the cores and in
turn the histotech often has problems seeing the cores in the paraffin

We are doing levels on these cases but find that often we need recuts
because we are not into the block enough.

I have heard of some techs who have used a little eosin in their 70%
ETOH, which helped the techs identify the cores.  I am reluctant to use
eosin on my automated processors in the 70% ETOH however.  We tried
dipping the blocks in eosin tinted formalin at the grossing stations.
The eosin leached out and did no good.   Does anyone have any good

I do know that some send eosin tinted formalin to sites that are doing
these biopsies.  I don't see that as an option for us.  Thanks for your

James R. Vickroy

Supervisor - MMC Surgical Pathology


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