Re: [Histonet] Manual processing

From:Maxim Peshkov

We have routine manually processing shedule for most specimens:
Fixation in 10% NBF overnight.
Dehydratation in acetone 5 x 30 min.
Intermediate step is acetone / white-spirit (substitute of xylene) 1:1 60 min
Clearing in white-spirit 4 x 30 min and leave here before morning.
Infiltration in paraffin 4 x 60 min, then embedding, cut, stain,
coverslip in this or next workday.
All procedures we do manually.

Best regards,

Maxim Peshkov
Histotechnologist, 
Taganrog 
Russia.


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