Re: [Histonet] IHC on Cheek cells
We used wooden tongue depressors to scrape cheeks for buccal smears - that
way you do not have cells trapped in cotton fibers. There are scrapers,
plastic that may work better, you can still resuspend the cells off the
ends - agitate the tubes containing scraper with a vortex mixer to let
cells go into PBS.
I think you could cytospin the cells onto slides easily.
At 09:45 AM 5/10/2006, you wrote:
> Hi, Histonet.
> I am staining phosoph-MEK on cheek cells. Here is the way how I
> collected cheek cells. I used cotton tips scrape inside of human
> mouth. then I soak the tip into the fixative offered by Thermo
> shandon for cytospin. Then I cytospined cells to slides.
> While I stained pMEK, I brought a rabbit IgG with it for negative
> control. I got a positive stain on cheek cells. Cells were very
> Anyone has IHC experience on buccal cells would like share with me, I
> really appreciated. Thanks a lot.
> Yan Gao
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Veterinary Molecular Biology
Montana State University - Bozeman
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Bozeman MT 59717-3610
406 994-4303 (FAX)
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