Yes, HPV typing can be performed on formalin-fixed, paraffin-embedded
tissue.  Although monoclonal antibodies to HPV type-specific proteins
exist, I recommend that you do in situ DNA hybridization for definitive
identification and typing.  I have used ProPath (Dallas, TX) and
Genzyme/Impath for this in the past.

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Paula Lucas"  05/12/06 11:38 AM >>>
Hello,

Could someone please advise us on HPV typing.

We received a request for HPV typing on tissue sent to us fixed in 10%
NBF
and our questions are:
can it be done on formalin fixed tissue, what is the procedure
(stain?), is
it sent out to what entity (a genzyme, a Unilab)?

Thanks,
Paula
Bio-Path Medical Group


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