Re: [Histonet] Good antibodies for macrophage detection in rat samples

From:Gayle Callis

F4-80 ( a rat antimouse monoclonal) is for mouse macrophages, not 
rat.  There are several well documented rat macrophage antibodies, go to 
Serotec and look for these.  ED1, ED2, ED3  are subsets.  These mouse 
antiRat  monoclonals were detected with goat antimouse secondaries that 
mouse IgG isotypes (IgG1, etc) biotinylated from Southern Biotechnology.

At 12:00 PM 5/18/2006, you wrote:
>Hello all,
>   We are interested in detecting macrophages in FFPE samples from rat. I=20
> would really appreciate any suggestions regarding the best antigen (Is 
> F4-80 the best?) and most useful commercial antibody to use.
>   Thanks in advance,
>   Guillermo
>LLama Gratis a cualquier PC del Mundo.
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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