Re: [Histonet] Good antibodies for macrophage detection in rat samples
F4-80 ( a rat antimouse monoclonal) is for mouse macrophages, not
rat. There are several well documented rat macrophage antibodies, go to
Serotec and look for these. ED1, ED2, ED3 are subsets. These mouse
antiRat monoclonals were detected with goat antimouse secondaries that
mouse IgG isotypes (IgG1, etc) biotinylated from Southern Biotechnology.
At 12:00 PM 5/18/2006, you wrote:
> We are interested in detecting macrophages in FFPE samples from rat. I=20
> would really appreciate any suggestions regarding the best antigen (Is
> F4-80 the best?) and most useful commercial antibody to use.
> Thanks in advance,
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Veterinary Molecular Biology
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