Re: [Histonet] FSGO

From:Gayle Callis

I used to have fish gelatin, a green gooey super thick liquid purchased 
from Sigma but it was not oily.

I have not used it for routine IHC non serum blocking step in lieu of BSA, 
a normal serum or casein blocking but some people in EM swear by it.

You may want to check up on some immuno-electron microscopy methods, that 
is where I have seen it used.

I know it was discussed many years ago on Histonet, a keyword search may 
bring it up.  Fish gelatin rather than fish skin gelatin.

  10:24 AM 5/8/2006, you wrote:
>For IHC purposes, is this Fish skin gelatin oil?   I have an IHC protocol
>which recommends using FSGO in PBS, although I'm not sure what it is or
>what it does.
>If you do, let me know.
>Jackie O'

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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