Re: [Histonet] FSGO
I used to have fish gelatin, a green gooey super thick liquid purchased
from Sigma but it was not oily.
I have not used it for routine IHC non serum blocking step in lieu of BSA,
a normal serum or casein blocking but some people in EM swear by it.
You may want to check up on some immuno-electron microscopy methods, that
is where I have seen it used.
I know it was discussed many years ago on Histonet, a keyword search may
bring it up. Fish gelatin rather than fish skin gelatin.
10:24 AM 5/8/2006, you wrote:
>For IHC purposes, is this Fish skin gelatin oil? I have an IHC protocol
>which recommends using FSGO in PBS, although I'm not sure what it is or
>what it does.
>If you do, let me know.
>
>Jackie O'
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
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