Re: [Histonet] Blocking endogenous peroxidase in pig LN

From:Gayle Callis

I have a peroxidase and pseudoperoxidase block that uses Glucose oxidase 
for complete removal of these endogenous problems!!!  It was originally 
used for an eosinophil study so I will attach to you privately.   We refer 
to it as GLUOX Block.  The method comes with references, you may want to 
access and read.

It works for minimally fixed frozen sections (acetone fixed) and also on 
FFPE tissues, and will not chew the frozen sections off the slide.

At 08:26 PM 5/14/2006, you wrote:
>Has anyone had experience with IHC on pig (hog) tissues?
>We are having trouble trying to block endogenous peroxidase in eosinophils 
>in frozen sections of lymph nodes.
>We have tried hydrogen peroxide up to 6% for one hour.
>Periodic acid at 0.23% for 1 minute helped but didn't remove all the 
>staining, longer time (up to 8 minutes) didn't improve the removal.
>Any help would be greatly appreciated.
>    Regards,      Laurie.
>Mr.Laurie Reilly
>School of Veterinary and Biomedical Sciences
>James Cook University
>Townsville. 4811
>Phone  07 4781 4468
>Fax      07 4779 1526
>Histonet mailing list

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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