Re: [Histonet] Blocking endogenous peroxidase in pig LN
I have a peroxidase and pseudoperoxidase block that uses Glucose oxidase
for complete removal of these endogenous problems!!! It was originally
used for an eosinophil study so I will attach to you privately. We refer
to it as GLUOX Block. The method comes with references, you may want to
access and read.
It works for minimally fixed frozen sections (acetone fixed) and also on
FFPE tissues, and will not chew the frozen sections off the slide.
At 08:26 PM 5/14/2006, you wrote:
>Has anyone had experience with IHC on pig (hog) tissues?
>We are having trouble trying to block endogenous peroxidase in eosinophils
>in frozen sections of lymph nodes.
>We have tried hydrogen peroxide up to 6% for one hour.
>Periodic acid at 0.23% for 1 minute helped but didn't remove all the
>staining, longer time (up to 8 minutes) didn't improve the removal.
>Any help would be greatly appreciated.
> Regards, Laurie.
>School of Veterinary and Biomedical Sciences
>James Cook University
>Phone 07 4781 4468
>Fax 07 4779 1526
>Histonet mailing list
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-4303 (FAX)
Histonet mailing list
<< Previous Message | Next Message >>