Re: [Histonet] Accidentally frozen NBF fixed tissue

From:Gayle Callis


Only when the NBF tissues were cryoprotected with sucrose before snap 
freezeing and never at -80C, always much colder.

There is a wonderful article in Histologic by Rena Fail and Vinnie Della 
Speranza on recovering poorly snap frozen biopsies where the ice crystal 
formation is obvious.  It was written last year or so.  Go to Sakura 
Finetek website,, click on Histologic and see how they did this.  It was 
easy and with good results on getting a decent section.

At 11:23 AM 5/3/2006, you wrote:
>Hello all,
>Someone in our lab accidentally froze 10% NBF fixed pancreas at -80
>degree C.
>They now want to get slides for H&E etc off that. Does anyone know
>whether this tissue is still usable after being frozen?
>Obviously it is not good to freeze fixed tissue, but has anyone ever
>done that and still gotten usable results? I would appreciate any input!

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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