Re: [Histonet] 34BE12

From:Patti Loykasek

    Hopefully I've understood your question & I'll try my best to answer
We do run a positive control for 34be12 - we use a normal prostate. Normal
prostate is not too difficult to obtain. We are usually running 34be12 on
several different cases covering different types of tissue. A separate
positive control slide enables us to grade the staining whether or not there
is any normal tissue staining on the patient material.  On all of our
positive controls we evaluate both the positive staining & the negative
staining - both must be appropriate for the QC to be considered acceptable
(this is stated in our SOP).
We run a negative control for the patient material (if there are enough
slides) to evaluate for any non-specific staining.
I hope this answers your question.

Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA

>  Gotta question for you ...
>  how  many  of  you  out  there  are  running a positive and a negative
>  control  for  your 34be12 on prostates?  How many are not?  How do you
>  handle  this in your procedure under your QC?  I have been asked to do
>  both.   Some  want  a  control  and  some  don't  I am trying to get a
>  concensus and be uniform.
>  The argument for no stains for normal tissue
>  The arguement for a still stains......
>  Any suggestions?
>  Roxanne
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