I use both BSA and normal serum from my secondary to block when I am 
staining both human and murine tissues with no problems. My BSA is 
molecular grade fraction, I am happy to share the catalog number 
should you need it.


>
>-----Original Message-----
>From: Lexus Ray [mailto:spicin82@hotmail.com]
>Sent: Tuesday, May 16, 2006 12:03 PM
>To: histonet@lists.utsouthwestern.edu
>Subject: [Histonet] blocking solution
>
>Hi,
>I'm a novice to immunolabeling.
>What are the advantages /disadvantages of using BSA in a blocking
>solution?
>Supposedly I used only normal sera in my blocking solution, would this
>result in high background resulting from primary antibody?
>
>L
>

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