RE: [Histonet] Protocols for estimating the swelling of tissue?

From:Kemlo Rogerson

I thought, and I stand to be corrected, that most fixatives caused shrinkage
of tissue. I assumed that to be due to the fixation, condensation, cross
linking of proteins or the eradication of water molecules. Hypertonic
sucrose would also cause shrinkage too as it would exert a negative osmotic
pressure on the 'more dilute' tissue, wouldn't it or have I got my pressures
all wrong.

Kemlo Rogerson
Pathology Manager
Ext  3311
DD   01934 647057
Mob 07749 754194


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-----Original Message-----
From: Ed Boyden [] 
Sent: Thursday, May 04, 2006 12:38 AM
Subject: [Histonet] Protocols for estimating the swelling of tissue?

Many procedures (fixation, immersion in sucrose, etc.) cause swelling
of tissue, versus the intact, original tissue.  Does anyone know of
 1. ways to predict the amount of swelling that occurs with a specific
protocol, or
 2. a protcol that would allow swelling of the tissue by a predictable

I would like to be able to compare samples done at different times,
and stored in different ways.

If not possible, I would like to be able to prepare new samples so
that their swelling will match my old estimates, as much as possible,
or at least that I can calculate the swelling of the new samples.

Thanks so much for your help!


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