RE: [Histonet] Marking prostate biopsies


We put hematoxylin on our cores at the grossing station.  We pull out the
cores, place them on a towel and use a pipette to place a drop of
hematoxylin on each core.  The cores are them placed between blue sponges
that have been soaked in formalin and them processed.  The cores are a nice
purple and show up well while embedding and while cutting and of course
doesn't interfere with staining.

Hope this helps,

-----Original Message-----
From: Vickroy, Jim [] 
Sent: Thursday, May 11, 2006 9:29 AM
Subject: [Histonet] Marking prostate biopsies


We are looking for a way to better mark our small needle biopsies such as
breast cores and prostate biopsies so that they are more easily seen after

Right now the embedder has some trouble identifying the cores and in turn
the histotech often has problems seeing the cores in the paraffin block.

We are doing levels on these cases but find that often we need recuts
because we are not into the block enough.


I have heard of some techs who have used a little eosin in their 70% ETOH,
which helped the techs identify the cores.  I am reluctant to use eosin on
my automated processors in the 70% ETOH however.  We tried dipping the
blocks in eosin tinted formalin at the grossing stations.
The eosin leached out and did no good.   Does anyone have any good


I do know that some send eosin tinted formalin to sites that are doing these
biopsies.  I don't see that as an option for us.  Thanks for your help.


James R. Vickroy

Supervisor - MMC Surgical Pathology



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