RE: [Histonet] IHC on Sakura Xpress processed tissue

From:"Bartlett, Jeanine \(CDC/NCID/VR\)"

Let me add that before we purchased the Sakura Xpress, I did a very involved study with the Milestone TT/Mega and the effects of microwave technology on IHC staining.  The IHC results were also excellent with this machine.

	-----Original Message----- 
	From: on behalf of Morken, Tim - Labvision 
	Sent: Thu 5/11/2006 4:14 PM 
	To: Morken, Tim - Labvision; 'Angela Bitting'; 
	Subject: RE: [Histonet] IHC on Sakura Xpress processed tissue

	I should mention tha the study below used an Energy Beam Sciences MWP 800
	microwave tissue processor, not the Sakura Xpress.
	-----Original Message-----
	[] On Behalf Of Morken, Tim
	- Labvision
	Sent: Thursday, May 11, 2006 12:37 PM
	To: 'Angela Bitting';
	Subject: RE: [Histonet] IHC on Sakura Xpress processed tissue
	Angela, You might want to look at this article in AJCP:
	Am J Clin Pathol. 2006 Feb;125(2):176-83.
	Related Articles, Links   
	A comparison of immunohistochemical stain quality in conventional and rapid
	microwave processed tissues.
	Emerson LL, Tripp SR, Baird BC, Layfield LJ, Rohr LR.
	Department of Pathology, University of Utah, Salt Lake City, USA.
	Same-day turnaround of pathology specimens is desirable in this era of
	managed care, and rapid microwave tissue processing produces histologic
	features of a quality equivalent to overnight processing. We studied whether
	microwave-assisted rapid tissue processing adversely affects the quality of
	immunohistochemical staining. We selected 30 specimens (20 neoplastic and 10
	nonneoplastic) from our routine surgical pathology workload. Paired large
	tissue blocks were made from each specimen type, one for microwave-assisted
	rapid processing and one for conventional processing. Two microarrays of 60
	punches each were made from the donor blocks. The microarray blocks were
	examined for intensity and extent of staining by 44 commonly used
	antibodies. Slides were reviewed independently by 2 pathologists blinded to
	the type of processing used. In 5,280 tissue punches examined, we found a
	high degree of concordance in quality, as measured by intensity and extent
	of immunohistochemical staining, between microwave and routinely processed
	tissues. Our study demonstrates that quality of immunohistochemical staining
	is similar between rapid microwave and conventional processing. The
	potential need for immunohistochemical analysis is not a contraindication
	for microwave-assisted rapid tissue processing.
	PMID: 16393680 [PubMed - indexed for MEDLINE]
	Tim Morken
	Lab Vision - Neomarkers
	Free webhosting for US State Histotechnology Societies:
	-----Original Message-----
	[] On Behalf Of Angela
	Sent: Thursday, May 11, 2006 11:14 AM
	Subject: [Histonet] IHC on Sakura Xpress processed tissue
	Can I get feedback from the Histonet world on how IHC staining is effected
	by switching from standard processing to processing on the Xpress? Does
	anyone see any other drawbacks to the technology?
	Angela Bitting, HT(ASCP)
	Technical Specialist, Histology
	Geisinger Medical Center
	100 N Academy Ave. MC 23-00
	Danville, PA 17822
	phone  570-214-9634
	fax  570-271-5916
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