RE: [Histonet] IHC on Sakura Xpress processed tissue

From:"Morken, Tim - Labvision"

I should mention tha the study below used an Energy Beam Sciences MWP 800
microwave tissue processor, not the Sakura Xpress.


-----Original Message-----
[] On Behalf Of Morken, Tim
- Labvision
Sent: Thursday, May 11, 2006 12:37 PM
To: 'Angela Bitting';
Subject: RE: [Histonet] IHC on Sakura Xpress processed tissue

Angela, You might want to look at this article in AJCP:

Am J Clin Pathol. 2006 Feb;125(2):176-83.
Related Articles, Links    
A comparison of immunohistochemical stain quality in conventional and rapid
microwave processed tissues.

Emerson LL, Tripp SR, Baird BC, Layfield LJ, Rohr LR.

Department of Pathology, University of Utah, Salt Lake City, USA.

Same-day turnaround of pathology specimens is desirable in this era of
managed care, and rapid microwave tissue processing produces histologic
features of a quality equivalent to overnight processing. We studied whether
microwave-assisted rapid tissue processing adversely affects the quality of
immunohistochemical staining. We selected 30 specimens (20 neoplastic and 10
nonneoplastic) from our routine surgical pathology workload. Paired large
tissue blocks were made from each specimen type, one for microwave-assisted
rapid processing and one for conventional processing. Two microarrays of 60
punches each were made from the donor blocks. The microarray blocks were
examined for intensity and extent of staining by 44 commonly used
antibodies. Slides were reviewed independently by 2 pathologists blinded to
the type of processing used. In 5,280 tissue punches examined, we found a
high degree of concordance in quality, as measured by intensity and extent
of immunohistochemical staining, between microwave and routinely processed
tissues. Our study demonstrates that quality of immunohistochemical staining
is similar between rapid microwave and conventional processing. The
potential need for immunohistochemical analysis is not a contraindication
for microwave-assisted rapid tissue processing.

PMID: 16393680 [PubMed - indexed for MEDLINE] 

Tim Morken
Lab Vision - Neomarkers

Free webhosting for US State Histotechnology Societies:

-----Original Message-----
[] On Behalf Of Angela
Sent: Thursday, May 11, 2006 11:14 AM
Subject: [Histonet] IHC on Sakura Xpress processed tissue

Can I get feedback from the Histonet world on how IHC staining is effected
by switching from standard processing to processing on the Xpress? Does
anyone see any other drawbacks to the technology?

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916

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