RE: [Histonet] IHC on Cheek cells

From:"Tony Henwood"

Rabbit antibodies are notorious for having a natural antibody population
directed against human keratins. I would recommend absorbing the tissues
with human keratin extracts. There was a paper some time ago (?10 years)
that described this. Unfortunately I can't find it (These days I have
problems finding my way home!!)


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
[] On Behalf Of yan gao
Sent: Thursday, 11 May 2006 1:46 AM
Subject: [Histonet] IHC on Cheek cells

   Hi, Histonet.

   I  am  staining  phosoph-MEK  on  cheek  cells.  Here is the way how
   collected  cheek  cells.   I  used  cotton tips scrape inside of
   mouth.   then  I  soak  the  tip  into  the fixative offered by
   shandon for cytospin.  Then I cytospined cells to slides.
   While  I  stained  pMEK,  I  brought a rabbit IgG with it for
   control.   I  got  a  positive  stain on cheek cells.  Cells were
   Anyone  has IHC experience on buccal cells would like share with me,
   really appreciated.  Thanks a lot.

   Yan Gao

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