F4/80 is a murine macrophage marker.  I use ED-1 from serotec, it’s a
mouse anti-rat macrophage marker.  It works well on FFPE tissue with
proteinase K digestion, (even works on formic acid decalcifed bone).

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz@premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309
 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Guillermo Palao
Sent: Thursday, May 18, 2006 12:01 PM
To: Histonet
Subject: [Histonet] Good antibodies for macrophage detection in rat
samples


Hello all,
   
  We are interested in detecting macrophages in FFPE samples from rat. I
would really appreciate any suggestions regarding the best antigen (Is
F4-80 the best?) and most useful commercial antibody to use.
   
  Thanks in advance,
   
  Guillermo

		
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