RE: [Histonet] FW: H&E on Leica XL automatic stainer

From:"Tony Henwood"

The following might be useful:

Program 1 - Routine H&E

Step	Station	Solution	Time	Exact
1	1	Xylene	4min	N
2	2	Xylene	4min	N
3	3	Ethanol	1min	N
4	4	Ethanol	1min	N
5	5	Ethanol	1min	N
6	Wash 1	Water	1½min	N
7	6	Haematoxylin	5min	Y
8	Wash 2	Water	2min	N
9	7	0.2% HCl	4sec	Y
10	Wash 3	Water	1min	N
11	8	Blueing	1min	N
12	Wash 4	Water	1min	N
13	9	Eosin	1min	Y
14	10	Ethanol	30sec	N
15	11	Ethanol	30sec	N
16	12	Ethanol	30sec	N
17	17	Xylene	1min	N
18	18	Xylene	1min	N
19	EXIT	Xylene		

Station Solutions
Station	Solution	Station	Solution
1	Xylene		10	Ethanol
2	Xylene		11	Ethanol
3	Ethanol		12	Ethanol
4	Ethanol		13	Ethanol
5	Ethanol		14	Ethanol
6	Haematoxylin	15	Ethanol
7	0.2% HCl in water	16	Spare
8	Blueing		17	Xylene
9	Eosin			18	Xylene
		EXIT	Xylene

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: [] On Behalf Of Sanders, Julie, VHACIN
Sent: Saturday, 6 May 2006 2:21 AM
Subject: [Histonet] FW: H&E on Leica XL automatic stainer

-----Original Message-----
From: Sanders, Julie, VHACIN 
Sent: Friday, May 05, 2006 11:28 AM
To: histonet@lists.utsouthwestern.ed; 200/316 Unmatched Invoices
Subject: H&E on Leica XL automatic stainer

Hi Netters,
I am trying to find different protocols for H&E on the Leica autostainer XL.  I would appreciate anyone using this stainer sharing their procedure.  Currently we are using Gills lll and Richard Allen Eosin...but I am interested in any/all procedures.  Feel free to email them to me privately if you wish. Thanks in advance, Julie

Julie A. Sanders, BA,HT(ASCP)
Supervisor, Anatomic Pathology
Cincinnati VAMC

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