[Histonet] specimen size for IHC

From:Heather Renko

The optimum size for IHC is 3-4mm thick and 1-2cm square to allow adequate penetration in the fixation process.  The Immunochemical Staining handbook(3rd Ed, published by Dako) states to cut at 4-6.  
   
  Heather Renko
   
   
  In your  invaluable, but,  no  doubt differing opinions, what  is  the  
optimal section thickness   for formalin fixed paraffin processed 
tissues for  immunostaining, using  DAB  as  chromogen?, there has been  
some  discussion here  whether  it  should be 4,5,6, or  7microns, many 
thanks.

                                                                                               
Richard  Edwards


		
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