[Histonet] safranin o staining- glycosaminoglycans-cartilage


Dear Histonetters,
                              I am in need of the procedure for staining of
glycosaminoglycans  in articular cartilage. The literature search indicated
the   use   of   safranin   o   staining   for   the   characterization  of
glycosaminoglycans.  If any body have good experience in this procedure and
able  to  share  their  protocol. I have already ordered a paper that deals
with this.
Method  of  Histomorphometric Assessment of Glycosaminoglycans in Articular
Journal of Orthopaedic Research Vol 15, 670-674.  1977
Choji Shimizu, Richard D. Coutts, Robert M. Healey Toshikazu Kubo, Yasusuke
Hirasawa and David Amiel.

Drug Discovery- Biology
Pre clinical Safety Assessment
Wockhardt Research Center, Aurangabad, India.
E.mail : Pkamalavenkatesh@wockhardtin.com

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