I have personally used eosin in the 95% step of the processor for years and it really helps with pinking up small cores and prostate bx's without having any effect on the final H&E.  We used the premade RA eosin.  I have used about 10-20ml in the Tissue Tek VIP which I believe?  is about a gallon or so of 95% in the carboy.  I started off with a little every time I would change the processor and gradually increased with no complaints from my pathologists.  Your embedder will thank you.
   
  Heather Renko, HT(ASCP)

		
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