Dear all,
I  would like to ask if anyone ever experienced fast bleaching of IF staining of your samples after the exposure to light emitted from the fluorescence microscope. I am staining with a FITC conjugated secondary Ab (Jackson Immunolab) and mounted with ProLong Gold (Molecular Probes). The fluorescence got very dim after 20-30sec of exposure. Though I got back the signal after removing it from the fluorescence microscope awhile but the signal was very much weaker from the very first time I saw in the microscope. I am unable to find out what is the cause. Can anyone please advise? 
Thanks alot!

With regards,
TIANG YEE PENG
Department of Medical Microbiology,
Faculty of Medicine,
University of Malaya, Malaysia
 
_________________________________________________________________
It’s the future of Hotmail: Try Windows Live Mail beta
http://www2.imagine-msn.com/minisites/mail/Default.aspx?locale=en-us_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet