[Histonet] RE: human-on-human IHC
A couple of days ago I wrote here about the Zenon rabbit-on-rabbit
kit. Well, there is also a human-on-human kit available from this
vendor (Molecular Probes/Invitrogen). It is based on a labeled (some
Alexa-fluorochromes, biotin, HRP) anti-human IgG Fab-fragment used for
the in vitro labeling of the humanized primary. Basically, the
technology behind is identical with the Dako ARKit.
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
NL-1105 AZ Amsterdam
Date: Mon, 1 May 2006 11:50:30 -0400
From: "Connolly, Brett M"
Subject: [Histonet] human-on-human IHC
I need advise on using human antibodies on human tissue. It will be an
unlabeled human primary.
i.e. what blocking protocol do you recommend...any other specific
you can point out?
Brett M. Connolly, Ph.D.
Merck & Co., Inc.
MRL, Imaging Research
PO Box 4
West Point, PA 19486
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