[Histonet] RE: goat primary Ab
So far I have seen just 2-step and 3-step streptavidin-biotin methods
coming by. We have tried here two biotin-free systems:
1) anti-goat polymer-HRP from Zymed/Invitrogen: it works nice with
clean background. However, the performance of this polymer is much
less compared with a 3-step streptavidin-biotin method (note:
anti-rabbit and -mouse polymers like EnVision+ and PowerVision are
nearly as sensitive as a 3-step streptavidin-biotin method).
2) 3-step approach with first rabbit anti-goat (Dako, 1/2500-5000, 30
min) followed by an anti-rabbit polymer-HRP. This is quite a sensitive
method that worked very good for us for a number of goat primaries.
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
NL-1105 AZ Amsterdam
Date: Tue, 16 May 2006 18:49:50 -0400
From: "Susan Bryant"
Subject: [Histonet] goat primary Ab
Hello to all
I am sending this for a friend in research....how many folks out in
Histoland are doing IHC with goat primaries on human, mouse and rat
tissues? What detection is everyone using, and are you getting
You may send your responses to me and I will print them for her.
Thanks for you time.
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