[Histonet] Protocols for estimating the swelling of tissue?

From:"Ed Boyden"

Many procedures (fixation, immersion in sucrose, etc.) cause swelling
of tissue, versus the intact, original tissue.  Does anyone know of
 1. ways to predict the amount of swelling that occurs with a specific
protocol, or
 2. a protcol that would allow swelling of the tissue by a predictable amount?

I would like to be able to compare samples done at different times,
and stored in different ways.

If not possible, I would like to be able to prepare new samples so
that their swelling will match my old estimates, as much as possible,
or at least that I can calculate the swelling of the new samples.

Thanks so much for your help!


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