[Histonet] Liver sections
Hello. I need some advice about cutting adult mouse livers. The livers are
fixed in paraformaldehyde overnight, rinsed, stored in 70% EtOH, and then
processed into paraffin. I usually cut at about 4um, but my sections look
like they are thicker...maybe 10um. Everything else I cut looks fine, itıs
just the liver. Usually I face the block and let it sit on ice for 1-2
hours before cutting. Sometimes if I am having trouble with shredding, I
will let it sit overnight on ice at 4°C. Any advice or suggestions on my
thickness problem would be greatly appreciated.
Thank you
Rachael
--
Rachael L. Emerson
Center for Pediatric Biomedical Research
University of Rochester Medical Center
575 Elmwood Avenue MRBX 1.11301
Rochester, NY 14642
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