[Histonet] FW: Jb-4 blocks

From:"Allen, Rhonda"

> Histonetter's,
> The following question was posted on the microscopy list serve by a
> friend.  I thought maybe some of the folks in histoland might have
> some good suggestions.
> Rhonda Allen
> "We are doing a rush job for a client who requires 4.0 um sections
> from JB 4 blocks.  Our ultramicrotomists extraordinaire, Cheryl, is
> having a dickens of a time getting the sections to remain flat when
> removing them from the knife.  She is cutting on glass and taking
> sections from the dry edge with a fine forceps.  As soon as the
> sections leave the knife, they curl and won't uncurl when placed on a
> drop of water on a slide. 
> Not only is this a rush job in support of a grant proposal, but it
> requires serial sectioning with no missing sections, and we have,
> like,
> no real experience with this resin.   Cheryl has tried various sized
> block faces and different thicknesses for the sections, but nothing is
> helping.
> The thumping sound you hear is a head hitting a wall----repeatedly.
> Can anyone HEEEELLLLP??"
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