I am just starting to work up the CD 10 Antibody (CALLA) Clone 56C6 from Novocastra.
I am testing at a range of 1:50, 1:100, 1:200 and 1:300 using EDTA antigen retrieval in a steamer for 20 minutes with 20 minutes cooling and 60 minutes in primary antibody on the Dako Autostainer with the Envision+ protocol.
Did any of you have any success with this antibody on renal cell carcinomas? My first efforts show only focal staining of the tumor in all the dilutions but 4+ internal control staining. My pathologists seem to expect more intense staining of the tumor cells.
Any suggestions would be very well received.
Thanks so much for listening.
Theresa Rohr, Nyack Hospital NY
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