[Histonet] Blocking endogenous peroxidase in pig LN
Has anyone had experience with IHC on pig (hog) tissues?
We are having trouble trying to block endogenous peroxidase in eosinophils
in frozen sections of lymph nodes.
We have tried hydrogen peroxide up to 6% for one hour.
Periodic acid at 0.23% for 1 minute helped but didn't remove all the
staining, longer time (up to 8 minutes) didn't improve the removal.
Any help would be greatly appreciated.
School of Veterinary and Biomedical Sciences
James Cook University
Phone 07 4781 4468
Fax 07 4779 1526
Histonet mailing list
<< Previous Message | Next Message >>