Re: [Histonet] GFAP - Rat
I also have had excellent results with DAKO's rabbit anti-cow GFAP
(Z0334). I use it with a Vector ABC Elite kit and DAB for the chromogen.
For FFPE sections my primary AB dilutions are about 1:5000 when using
nickel + cobalt to intensify the DAB, more concentrated without the
nickel-cobalt.
Geoff
Bruijntjes, J.P. wrote:
>Hi all
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>Is anyone of you working with rat brain and GFAP?
>
>I have noticed that many GFAP antibodies do cross react with rat tissue,
>but maybe someone of you is working with rat tissue and does have a
>preference to a particular antibody. If so, can you share this knowledge
>with me?
>
>
>
>Joost Bruijntjes
>
>TNO Quality of Life
>
>Zeist Holland
>
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>This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html
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--
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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