Re: [Histonet] Frozen TMA's


We have direct experience with a lab that prepared frozen tissue arrays. 
However, the  quality of the section of cores was very poor until they used 
the CryoJane Tape-Transfer system. The morphology was preserved. Sectioning 
the block did not raise the problems you describe.

See publication, M .Schoenberg- Fejzo and Dennis J. Slamen "Frozen Tumor 
Tissue Microarray Technology  for Analysis of Tumor RNA, DNA, and Proteins, 
. American Journal of Pathology November, 2001,
pg 1645. The paper discusses the CryoJane and says "The tape transfer system 
(Instrumedics, Inc)
was critical to maintaining the integrity of the sample"

Please visit our web site and click on "products" and go to "CryoJane"  to 
see how the system works!


----- Original Message ----- 
From: "Thomas Crowell" 
Sent: Wednesday, June 01, 2005 1:34 PM
Subject: [Histonet] Frozen TMA's

> Hello all,
> I'm hoping that there are some experts among the histonetters who have
> mastered the technique of creating frozen tissue microarray blocks, and
> would be willing to share some of the details with our laboratory.  We are
> currently using a 2.5mm Harris uni-core to make a 4X5 template, and using
> the same core size to sample tissues - the cores fit nicely into the
> templates, however the interface between the tissue core and the OCT block
> is creating much havoc in trying to obtain reproducible sequential
> cryosections (sections curl up, drag along the blade, compress).  Smearing
> a layer of OCT onto the block surface does help to fill in the interface
> irregularities, but only alleviates the problem for 3- 5 sections.
> Any suggestions for improvement would be greatly appreciated!
> Tom Crowell
> BiogenIdec
> Cambridge, MA

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