RE: [Histonet] substitute for xylene

Hi Myriam and Ruth,

If you have access to a vibrating microtome, you might be able to section
the polystyrene without embedding it.

Best wishes,


                      "Yaskovich, Ruth A                                                                                   
                      (NIH/NIDCR)"                          To:, "''"     
                      Sent by:                              cc:       (bcc: Jan Minshew/USDER/West/Leica)                  
                      histonet-bounces@lists.utsouth        Subject:  RE: [Histonet] substitute for xylene                 
                      05/27/2005 08:18 AM                                                                                  

If your samples are just the polystyrene without tissue just use the MMA
hand process. You really don't need any xylene. I have done it before with
no problems.
Ruth Yaskovich
National Institutes of Health
Pain Neurosensory Mechanism Branch
Drug Discovery

> ----------
> From:  
> Sent:            Friday, May 27, 2005 3:46 AM
> To:    
> Subject:         [Histonet] substitute for xylene
> Hello everyone
> i have some samples made of polystyrene and i want to embed them in
> paraffin or MMA. But xylene and toluene attacks polystyrene. DO you Know
> another clearing agent to replace Xylene ?
> Thank you very much in advance.
> Myriam baali
> natural implant
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