Hi Myriam and Ruth,

If you have access to a vibrating microtome, you might be able to section
the polystyrene without embedding it.

Best wishes,

Jan



                                                                                                                           
                      "Yaskovich, Ruth A                                                                                   
                      (NIH/NIDCR)"                          To:       histonet@pathology.swmed.edu, "'Myri37@aol.com'"     
                                                                             
                      Sent by:                              cc:       (bcc: Jan Minshew/USDER/West/Leica)                  
                      histonet-bounces@lists.utsouth        Subject:  RE: [Histonet] substitute for xylene                 
                      western.edu                                                                                          
                                                                                                                           
                                                                                                                           
                      05/27/2005 08:18 AM                                                                                  
                                                                                                                           
                                                                                                                           




Myriam,
If your samples are just the polystyrene without tissue just use the MMA
and
hand process. You really don't need any xylene. I have done it before with
no problems.
Ruth Yaskovich
National Institutes of Health
N.I.D.C.R.
Pain Neurosensory Mechanism Branch
Drug Discovery

> ----------
> From:            Myri37@aol.com
> Sent:            Friday, May 27, 2005 3:46 AM
> To:              histonet@pathology.swmed.edu
> Subject:         [Histonet] substitute for xylene
>
> Hello everyone
>
> i have some samples made of polystyrene and i want to embed them in
> paraffin or MMA. But xylene and toluene attacks polystyrene. DO you Know
> another clearing agent to replace Xylene ?
> Thank you very much in advance.
>
> Myriam baali
> natural implant
>
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> Histonet@lists.utsouthwestern.edu
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>

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