RE: [Histonet] (no subject)

From:"Favara, Cynthia (NIH/NIAID)"


Some pertinent questions would be:
	How are you collecting the sample?
	What is the processing schedule?

	I have done some studies here with mouse mammary glands and have
collected the gland including the nipple. I have not had any problems with

We shaved the belly being careful around the nipple so as not to injure the
tissue. Cut a thin section on either side of the nipple. Process using ETOH
70%-2 hours, 80% -1 hour, 2 95% ETOH - 20 minutes each, 2 100% ETOH 20
minutes each, 3-Propar 30 minutes each, 4 paraffins 30 minutes each. Embedd
as you would skin. In my hands most mouse tissue requires some soaking prior
to cutting. Be sure the temp of your water bath is not to hot or the fat
beneath the skin will just explode. I find the temperature range to be
fairly narrow.

Hope this is helpful.



Cynthia Favara
903 South 4th Street
Hamilton, MT 59840

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-----Original Message-----
From: Margaryan, Naira [] 
Sent: Monday, May 23, 2005 11:35 AM
To: histonet
Subject: [Histonet] (no subject)

Hi Histos!


I am having problems with cutting FFPE mouse nipple. Paraffin is cutting
well but small tissue just crushing. We can't collect samples.

Please help, any suggestion is appreciated.





Naira V. Margaryan, Ph.D, D.V.M.

Research Associate II

Children's Memorial Research Center

2300 Children's Plaza, Box 222

Chicago, IL 60614-3394

Tel: 773-880-4000/5-6740

Fax: 773-755-6594


For Express Mail: 

CMRC, Room C.473

2430 N. Halsted Street

Chicago, IL  60614-4314


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