Along those same lines ... for staining CD31/PECAM in the melanoma 
samples I work with I use True Blue chromagen and it works 
beautifully!

Andrea

At 7:25 AM -0500 6/14/05, Dawson, Glen wrote:
>Jane,
>
>I've found that a melanin bleach hurts IHC staining.  Have you considered
>using something like Nova Red from Vector as a good DAB substitute that
>yields red staining?  This red chromagen contrasts much better with the
>melanin in the tissue and I've found I don't have to modify any of my usual
>procedures to achieve good staining.  It is also permanent and does not wash
>out in alcohols like say an alc. phos. red.  Let me know if you need the
>catalogue #.
>
>Good Luck,
>
>Glen Dawson  BS, HT & QIHC (ASCP)
>IHC Manager
>Milwaukee, WI

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