RE: [Histonet] melanin renoval

From:"Dawson, Glen"


I've found that a melanin bleach hurts IHC staining.  Have you considered
using something like Nova Red from Vector as a good DAB substitute that
yields red staining?  This red chromagen contrasts much better with the
melanin in the tissue and I've found I don't have to modify any of my usual
procedures to achieve good staining.  It is also permanent and does not wash
out in alcohols like say an alc. phos. red.  Let me know if you need the
catalogue #.

Good Luck,

Glen Dawson  BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI

-----Original Message-----
From: Jane Moose []
Sent: Monday, June 13, 2005 3:53 PM
Subject: [Histonet] melanin renoval

Does anyone have a procedure to bleach unstained slides (from FFPE tissue)
in order to remove melanin
in order to stain with DAB for  PECAM?

Jane Moose
LIS Coordinator/Histology
Newberry County Memorial Hospital
Newberry,  SC

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