RE: [Histonet] melanin renoval

From:"Jackie M O'Connor"

Or how about a red or purple DAB replacement?  Biocare has a couple of 
permanent chromogens that can be used for HRP.  I plan to do exactly this 
for some melanin-loaded xenografts.

Jacqueline M. O'Connor HT(ASCP) QIHC
Assistant Scientist
GPRD Cancer Research
Abbott Laboratories, Abbott Park, IL

"Elizabeth Chlipala" 
Sent by:
06/13/2005 02:58 PM

        To:     "'Jane Moose'" , 

        Subject:        RE: [Histonet] melanin renoval


Rather than perform a melanin bleach why don't you switch to a alkaline
phosphatase detection system and use fast red as your chromagen.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
[] On Behalf Of Jane
Sent: Monday, June 13, 2005 2:53 PM
Subject: [Histonet] melanin renoval

Does anyone have a procedure to bleach unstained slides (from FFPE
tissue) in order to remove melanin
in order to stain with DAB for  PECAM?

Jane Moose
LIS Coordinator/Histology
Newberry County Memorial Hospital
Newberry,  SC

This message is intended only for the use of the individual or entity to
which it is addressed and may contain information that is privileged,
confidential and exempt from disclosure under applicable law.  If the
of this message is not the intended recipient, you are hereby notified
any dissemination, distribution, or copying of this communication is
strictly prohibited by law.  If you have received this communication in
error, please notify me immediately.
Histonet mailing list

Histonet mailing list

Histonet mailing list

<< Previous Message | Next Message >>