RE: [Histonet] NADH stain

From:Tony Henwood


The NADH probably refers to the enzymatic demonstration of Nicotinic Acid
Adenine Dinucleotide Dehydrogenase. It is used for the demonstration of
mithochondria especially in frozen sections of muscle biopsies.

The principle of the stain: The oxidation of NADH can be catalysed by flavin
enzymes (formerly called diaphorases). NADH-TR is a flavin enzyme which
takes up hydrogen reversibly and transfers it to the acceptor, a tetrazolium
salt. The tetrazolium is converted to purple-blue formazan pigment marking
the site of enzyme activity.


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: Patsy Ruegg [] 
Sent: Thursday, 26 May 2005 4:13 AM
To: 'Histonet'
Subject: [Histonet] NADH stain

A pathologist is asking for a stain, possibly IHC he is calling NADH, does
this mean anything to anyone?
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