RE: [Histonet] Ki67.

From:"Bruijntjes, J.P."

Hi Christian

Yes I do. May be it depends on the antibody you use.=20We use a rabbit
monoclonal ki67 from Neomarkers (Labvision). Tissues fixed in alcohol
don't react with the antibody using HIER in citrate buffer (pH 6,0). At
least it didn't in my lab. It does when you use HIER=20in EDTA (pH 8.0)
and in TRIS (pH 10). 
Hopes that will help you

Joost Bruijntjes
TNO Quality of Life

-----Original Message-----
[] On Behalf Of
Sent: woensdag 1 juni 2005 9:51
Subject: [Histonet] Ki67.

Hey users of histonet.
I am trying to stain kidneys for ki67!
The sections have been in alcohol for 1 month and are now in parafin.
The antibody I am using should be good enough.
Does anyone have experience with this???
best regards. Christian EIsbo.

Histonet mailing list

This e-mail and its contents are subject to the DISCLAIMER at

Histonet mailing list

<< Previous Message | Next Message >>